The Type VI secretion system (T6SS) is a molecular weapon used by bacteria to inject toxic proteins into rival cells. While well studied in some species, its function in Acinetobacter baylyi is less understood. This project explored T6SS-related genes by using Pseudomonas aeruginosa as a competitor to select mutant strains. These were analysed using competition assays, live-cell microscopy, and genome sequencing. Key findings highlight the importance of VipA / VipB interaction and the presence of TslA in assembly as well as the essential role of ClpV in disassembly of the structure. The results provide insights into the function of the T6SS bacteria, contributing to our understanding of how bacteria compete and survive.