Facing the global health crisis of antimicrobial resistance, my research tackles a critical defense mechanism in E. coli. This project is dedicated to engineering a new pathway to more effective and sustainable treatments for persistent bacterial infections. E. coli, is a widespread Gram-negative pathogen and frequently causes severe conditions from UTIs to sepsis. While antibiotics are the frontline, this bacterium has evolved formidable resistance. One of the mechanisms that helps E. coli resist these antibiotics are efflux pumps, they actively expel these drugs. My investigation centers on the pivotal role of these pumps, proposing an innovative strategy: their inhibition. By exploring how disrupting efflux pump function under varying acidic conditions impacts bacterial vulnerability.