Neuropathic pain affects over half of chemotherapy patients, yet effective treatments remain limited. This study focused on the surroundings of the choroid plexus, hypothesizing that its immune cells influence ependymal macrophages via cytokines. Using a rat model of paclitaxel-induced neuropathy, proinflammatory (activated, ED1+) and anti-inflammatory (resident, ED2+) macrophages in the ependymal layer were labeled by immunofluorescence. Microscopy was used for visualization and quantification per mm². Results showed an early rise in ED1+ cells, followed by the occurrence of the ED2+ compensatory mechanism by day 7. Findings suggest a dynamic immune response and help to clarify the potential role of the choroid plexus in chemotherapy-induced neuroinflammation.